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OK O antisera are suitable for screening for surface antigens on live cultures, and are particularly suited to test for known EPEC and/or STEC (Shiga toxin-producing E.coli, also known as VTEC: Verocytotoxin-producing E.coli) serotypes.

A positive agglutination test is not indicative of the presence of relevant virulence genes. Positive isolates should therefore be investigated further for virulence factors.    

 

E. COLI OK O ANTISERUM FOR LIVE CULTURE

OK O antisera are polyclonal antisera from rabbits. Serum contains antibodies against several surface antigens . For this reason OK O antisera are intended only for screening and must always be confirmed with O serotyping. OK O antisera are supplied in dropper bottles of 3 mL ready-to-use antiserum, sufficient for at least 150 reactions.

Place a small drop (approximately 20 μL) of antisera on a glass slide. Transfer culture from two to three colonies to the drop of antiserum and mix well. A positive result will be visible to the unaided eye as agglutination within 10 seconds. Positive slide results need to be confirmed with O antisera for boiled culture. 

OK O pool antisera

Article number Product React with antigen Number of tests Packing 
44292 Pool 1 EPEC/VTEC/ STEC O26, O103, O111, O145, O157 150 3 mL vial 
44293 Pool 2 EPEC O55, O119, O125ac, O127, O128ab 150 3 mL vial 
77713 Pool 3 EPEC O86, O114, O121, O126, O142 150 3 mL vial 

OK O single antisera

Article number Product Incl. in pool Number of tests Packing 
 44295 O26 150 3 mL vial 
 53085 O45  150 3 mL vial 
 44296 O55 150 3 mL vial 
 44297 O86 150 3 mL vial 
 44298 O103 150 3 mL vial 
 44299 O111 150 3 mL vial 
 44300 O114 150 3 mL vial 
 44301 O119 150 3 mL vial 
 44302 O121 150 3 mL vial 
 44303 O125ac 150 3 mL vial 
 44304 O126 150 3 mL vial 
 44305 O127 150 3 mL vial 
 44306 O128ab 150 3 mL vial 
R* 72682 O138  
R* 72686 O139  
 44307 O142 150 3 mL vial 
 44308 O145 150 3 mL vial 
R* 72687 O149  
 44309 O157 150 3 mL vial 
R* 44310 O158  

*Please note that the products marked with an R are restricted, indicating the potential need for a forecast, minimum order or extra fee). Please contact us via the contact form if you have an inquiry for one of these products.  

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