E. coli antisera overview
Over the past decades, a number of new several clinically relevant E. coli pathogens have been identified, e.g. EPEC (enteropathogenic E. coli), STEC (shigatoxin-producing E. coli) , also known as VTEC (verocytotoxin-producing E. coli).
These discoveries have reinforced the need for E. coli serotyping, as an important tool in diagnosis, monitoring and testing. Antisera from SSI Diagnostica Group are for in vitro use in human, veterinary and food laboratories
All antisera are produced in accordance with internationally recognized methods dating back to the time of Fritz Kauffmann, Ida Ørskov and Frits Ørskov, combined with the knowledge accumulated since then.
Our comprehensive antisera range enables serotyping of all known E. coli strains — the gold standard method for accurate phenotypic classification.
Product overview
All antisera are polyclonal and are produced in rabbits with well-defined reference strains. All finished products are tested against a large number of strains.
| Product | Targets | When to use (primary intent) |
|---|---|---|
| OK O Pool – STEC / VTEC Big Six | O antigens (LPS) O26, O45, O103, O111, O121, O145 | Rapid screening for Big Six non‑O157 STEC / VTEC (confirm with O antisera on boiled culture) |
| OK O antisera (single and pools) | Most prevalent EPEC / STEC/ VTEC O antigens (LPS) | Rapid screening of the most prevalent EPEC/ STEC/ VTEC on live culture (confirm with O antisera on boiled culture) |
| O antisera (single & pools) | All188 O-antigens (LPS) via single and pool antisera | Full serotyping (confirmatory testing on boiled culture) |
| H antisera (single and pools) | All 53 H antigens (Flagella proteins) | Defining H component of serotype (e.g., O157:H7) |
| K antisera (RUO) | All 67 K antigens (Capsular polysaccharides) | Additional capsule typing of serotype if needed alongside O/H results |
| F4 & F5 antisera (fimbriae) | F antigens (Fimbrial adhesins) — ETEC | Additional typing for F4 and F5 related ETEC investigations |
Please note: some of our E. coli antisera are partly restricted, indicating the potential need for a forecast, minimum order or extra fee. Specific information can be found on the respective product pages. All products are CE IVD marked except our K antisera which are RUO.
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FAQ:
SSI Diagnostica Group cannot guarantee that antisera will continue to meet their specificity and sensitivity as well as performance requirements after being frozen.
The antisera are supplied ready to use. Because they contain polyclonal antibodies, they are quality-controlled based on antibody performance rather than a specified antibody concentration.
No. Ready-to-use antisera are supplied for direct use. They should not be diluted further, as this will affect their performance.
The antisera are polyclonal rabbit sera and are not classified as exclusively IgG or IgM.