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E. coli F4 and F5 antisera
Fimbrial adhesins F4 and F5 (F antigens) help enterotoxigenic E. coli (ETEC) to bind to the intestinal mucosa.
By this means they can severely disrupt intestinal function and cause disease in the form of diarrhoea in infants and newborn piglets.
Product description
F antisera are polyclonal antisera produced by immunizing rabbits. Previously, F4 and F5 were named K88 and K99 and it was wrongly thought that they were capsule antigens. Today, K88 and K99 no longer exist.
How to use
F antisera are used for slide agglutination with living cultures.
Place a small drop (approximately 20 μL) of antisera on a glass slide. Transfer culture from two to three colonies to the drop of antiserum and mix well. A positive result will be visible to the unaided eye as agglutination within 10 seconds.
Product range
Please note that both F4 and F5 antisera products are restricted (also indicated by R mark in the table), indicating the potential need for a forecast, minimum order or extra fee. Please contact us via the contact form if you have an inquiry for one of these products.
| Article number | Product | |
|---|---|---|
| R | 51172 | F4 fimbrial antisera for typing of pili |
| R | 51173 | F5 fimbrial antisera for typing of pili |
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